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Quick Reference

  • Code definition: 84484 reports a quantitative blood assay measuring cardiac troponin concentration (I or T isoform); any assay returning a numeric result uses this code, regardless of analyzer platform or isoform measured.
  • Key billing rule: MUE = 2 per date of service under Medicare [5]; each unit requires independent clinical justification, and Modifier 91 is required on repeat draws performed the same day.
  • Modifier essentials: Modifier 91 for serial same-day draws; Modifier 90 when a reference laboratory performs the assay and the billing provider collects the specimen. PC/TC Modifier (26/TC) does not apply (indicator = 9).
  • Documentation must-have: The ordering note must record the specific clinical indication (e.g., "acute chest pain, rule out NSTEMI") for each draw; a blanket protocol order without patient-specific context is insufficient for audit purposes [3].
  • Top confusion point: 84512 (troponin, qualitative) is not interchangeable with 84484. If the device reports only positive/negative, 84512 is correct; 84484 requires a numeric concentration result.
  • Payer alert: 84484 is paid under the Clinical Laboratory Fee Schedule (CLFS), not the Physician Fee Schedule. In hospital outpatient settings, the APC Status Indicator "Conditionally packaged laboratory tests" means 84484 may not be separately payable when billed the same day as a significant procedure [3].
  • CLIA requirement: 84484 is NOT CLIA waived [8]; the performing laboratory must hold a Certificate of Compliance or Accreditation for moderate or high complexity testing. Modifier QW is never appropriate for this code.

When to Use This Code

84484 captures any quantitative troponin assay that reports a numeric concentration from serum or plasma. There is no separate CPT code distinguishing troponin I from troponin T at the quantitative assay level; both isoforms measured quantitatively are reported with 84484 [2]. The ACC/AHA 2021 Chest Pain Guideline designates high-sensitivity cardiac troponin (hs-cTn) as the preferred biomarker for ACS evaluation, recommending Class I serial algorithms at 0/1h or 0/2h for early NSTEMI rule out [1]. As of CPT 2026, no distinct code has been established to separate high-sensitivity from conventional troponin assays; both are reported with 84484 [2].

Primary clinical indications:

  • Acute chest pain with suspected ACS or NSTEMI, including serial draws per 0h/3h or 0h/1h rapid rule out protocols
  • Suspected STEMI (initial and confirmatory testing)
  • Pulmonary embolism workup, where troponin elevation signals right ventricular strain
  • Myocarditis or pericarditis evaluation
  • Sepsis-associated myocardial injury monitoring
  • Heart failure exacerbation (to differentiate from ACS and assess prognosis)
  • Perioperative cardiac monitoring (post-CABG, post-PCI, or high-risk noncardiac surgery)
  • Chemotherapy cardiotoxicity surveillance (anthracycline-based regimens)
  • Cardiac contusion following blunt chest trauma

The code selection criterion is result format, not device type or clinical setting: a numeric concentration in any unit (ng/mL, pg/mL, etc.) equals 84484; a binary positive/negative result equals 84512. This applies whether the analyzer is in a hospital core laboratory, a clinic, or a point-of-care device.


Code Differentiation Table

Code Description When to Use Instead
84484 Troponin, quantitative Any numeric concentration result (ng/mL, pg/mL) for any clinical indication
84512 Troponin, qualitative Point-of-care device reports positive/negative only; result is not a numeric value
82553 Creatine kinase (CK), MB fraction CK-MB ordered alongside troponin for timing differentiation or protocol; separately billable
83880 Natriuretic peptide (BNP/NT-proBNP) Dyspnea evaluation to differentiate heart failure from ACS; separately billable
83874 Myoglobin Early rising cardiac marker ordered as adjunct; less specific than troponin

The critical differentiator between 84484 and 84512 is the assay result format alone. A bedside analyzer that generates a numeric troponin value is 84484 even if used at a small clinic or urgent care; a lateral flow strip that returns "POSITIVE" is 84512 even if performed in a hospital emergency department. Billing 84484 when the result is qualitative misrepresents the service performed.


Billing & Modifier Rules

Modifier 91 (Repeat Clinical Diagnostic Laboratory Test): When 84484 is ordered multiple times on the same date, each repeat draw intended to generate independent clinical results requires Modifier 91 on the second and any subsequent units [3]. Modifier 91 is not appropriate when a repeat is performed to confirm an initial result due to equipment malfunction, specimen quality issues, or any quality control purpose. It is inapplicable when another code inherently describes a series of results (not applicable here). The modifier applies only to laboratory tests performed more than once on the same calendar date for the same patient [3].

MUE = 2: Medicare allows a maximum of 2 units of 84484 per claim line per date of service [5]. This ceiling aligns with the standard 0h/3h serial protocol. If a three-draw protocol (0h/3h/6h) is clinically documented, the third unit will trigger automated MUE review; coders should consult MAC-specific guidance on whether line-level MUE versus date-of-service MUE applies and whether separate line submission with draw times supports additional payment.

Modifier 90 (Reference Laboratory): Append Modifier 90 when the billing provider collects the specimen but sends it to an outside reference laboratory for analysis. Modifier 90 is uncommon for stat troponin (typically performed in house) but applies in settings without on-site laboratory capability.

Modifier 26/TC: PC/TC indicator = 9 for 84484; professional and technical components are not separately reportable. Do not append Modifier 26 or TC to chemistry analyte codes.

Modifier QW (CLIA Waived): 84484 is not CLIA waived [8]. Do not append QW. For qualitative troponin on a CLIA-waived device, use 84512 with Modifier QW after verifying the specific device's waived status on the CDC CLIA waived test list [9].

Venipuncture: Specimen collection (36415) is separately reportable and is not bundled with chemistry analyte codes [2].

NCCI PTP edits: 84484 has limited procedure-to-procedure bundling exposure as a standalone analyte code. It is not bundled into a comprehensive metabolic panel or cardiac panel in standard CPT. Verify current-quarter PTP edit tables for any new edit pairs [4].


Documentation Essentials

For each unit of 84484 billed, the medical record must support:

  • Clinical indication: The ordering note must document the specific reason for the test (e.g., "chest pain with radiation to left arm, rule out NSTEMI," not simply "cardiac labs"). Routine screening without symptoms does not satisfy Medicare medical necessity under SSA 1862(a)(1)(A) [3].
  • Physician order: A signed or electronic order with the ordering provider's NPI. For serial draws, each repeat should be ordered or explicitly authorized; a single standing protocol order without patient-specific context is insufficient for audit purposes.
  • Serial draw justification: For Modifier 91 claims, documentation must explain why the repeat draw was clinically necessary (e.g., "3-hour serial troponin per ACS protocol, initial result borderline at 0.04 ng/mL"). Auditors specifically look for serial draw documentation when Modifier 91 is billed.
  • Performing laboratory NPI: Must appear on the claim. When Modifier 90 is appended, both the billing entity and the performing entity must be identifiable.
  • Result interpretation: Troponin results should be referenced or interpreted in the clinical note. Orders with no documented follow-up or clinical interpretation are a high-volume audit flag for cardiac biomarker testing [10].

Audit red flags specific to 84484:

  • Two or more units billed without Modifier 91, triggering duplicate claim logic
  • Diagnosis codes that do not clinically support troponin testing (e.g., isolated musculoskeletal back pain with no cardiac symptoms)
  • Claims from facilities without appropriate CLIA certification for moderate or high complexity testing [8]
  • Serial troponin billed for patients with no documented cardiac or ischemic symptoms

Medicare, Commercial & Medicaid Payer Rules

Medicare:

84484 is paid under the CLFS, not the MPFS [7]. The Statutory Exclusion status means no geographic GPCI adjustment applies; payment is uniform nationally. There is no National Coverage Determination specific to troponin testing [6]; coverage is adjudicated under the general laboratory medical necessity standard (SSA 1862(a)(1)(A)). Individual MACs may publish LCDs or Articles covering cardiac biomarker testing; verify the applicable MAC portal for jurisdiction-specific guidance before assuming coverage.

In hospital outpatient settings, the APC Status Indicator "Conditionally packaged laboratory tests" means 84484 may not be separately payable when performed on the same day as a significant diagnostic or surgical procedure under OPPS packaging rules. This is an expected OPPS policy outcome, not a billing error. Independent laboratories and physician offices are not subject to APC packaging.

MUE = 2 is enforced by Medicare automated edits at the claim line level [5]. Appeals for additional units require medical record documentation supporting each draw independently.

Commercial Payers:

Commercial payer policies generally align with Medicare's medical necessity standard for troponin, but prior authorization requirements, diagnosis restrictions, and unit limits vary by plan. The high frequency of Modifier 90 usage in 84484 claims (approximately 62% per modifier utilization data) indicates that reference laboratory billing is common for this code; plan-specific reference laboratory policies and network status directly affect payment. Some commercial payers bundle serial troponin draws into an emergency department visit payment; verify the applicable plan's laboratory policy before assuming separate payment for each unit.

Medicaid:

State Medicaid programs and managed Medicaid plans vary in laboratory fee schedule rates and serial draw coverage. Some states require prior authorization for repeated inpatient laboratory testing. No uniform federal Medicaid coverage standard applies to 84484 specifically; verify the applicable state or managed care plan policy.


Common Denials & Prevention

Duplicate claim denial (Modifier 91 missing) When 84484 is billed twice on the same date without Modifier 91, payer systems flag the second unit as a duplicate claim submission. This is the most common denial pattern for serial troponin protocols. Prevention: Append Modifier 91 to the second and any subsequent unit of 84484 on the same date. The clinical note must document the reason for each repeat draw; "serial troponin per protocol" is sufficient only when supported by patient-specific context.

MUE exceeded (more than 2 units) Billing three or more units of 84484 on the same date triggers automatic denial under the Medicare MUE = 2 limit [5]. Prevention: If a three-draw protocol is clinically documented, consult MAC-specific guidance on line-level versus date-of-service MUE adjudication. Denials may be appealed with documentation showing the specific draw times and independent clinical necessity for each specimen.

Medical necessity denial (unsupported diagnosis) Claims submitted with diagnosis codes that do not support troponin testing (e.g., chronic musculoskeletal pain only) are denied under general lab medical necessity rules [3]. Prevention: The primary diagnosis on the claim should reflect the indication for the troponin order. Use presenting symptom codes (e.g., R07.9 for chest pain, unspecified) when a definitive diagnosis has not been established at the time of ordering.

Incorrect code selection (84484 vs 84512) Defaulting to 84484 for all troponin tests, including qualitative point-of-care devices that report positive/negative only, results in misrepresentation of the service performed. Prevention: Verify the laboratory report format before code selection. A numeric value in any unit confirms 84484. A binary result confirms 84512.

CLIA certificate mismatch Billing 84484 from a facility holding only a Certificate of Waiver is improper; quantitative troponin requires moderate or high complexity CLIA certification [8]. Prevention: Confirm the performing laboratory's CLIA certificate type before billing. Certificate of Waiver facilities are limited to CLIA-waived tests only. Standard quantitative troponin analyzers require a Certificate of Compliance or Accreditation.


Coding Scenarios

Scenario 1: Emergency Department Serial Troponin, ACS Rule Out

A 58-year-old presents to the emergency department with substernal chest pain and diaphoresis. The physician orders a troponin per the ACS protocol; two quantitative draws are performed at 0 hours and 3 hours, returning 0.02 ng/mL (normal) and 0.18 ng/mL (elevated), prompting an NSTEMI diagnosis.

Correct coding: 84484 x 2 units; Modifier 91 on the second unit; ICD-10-CM I24.9 (NSTEMI, after second draw confirms) or R07.9 for the initial presentation.

Why: Both draws return numeric concentrations; 84484 is correct for both. Modifier 91 on the second unit signals an intentional repeat for independent clinical results and prevents a duplicate-claim denial. MUE = 2 is satisfied without additional documentation.

Scenario 2: Point-of-Care Qualitative Troponin at a Rural Clinic

A rural urgent care clinic uses a lateral flow point-of-care device that reports troponin as "POSITIVE" or "NEGATIVE" only. The device is listed on the CMS CLIA waived test list. A patient presents with atypical chest pain and the test returns "NEGATIVE."

Correct coding: 84512 with Modifier QW; ICD-10-CM R07.9.

Why: The result is qualitative (positive/negative), not a numeric concentration, so 84484 is incorrect. Modifier QW is appropriate only because this specific device holds CLIA-waived status confirmed on the CDC waived test list [9]; verify the device before appending QW.

Scenario 3: Inpatient Myocarditis Workup with Multiple Cardiac Biomarkers

A 32-year-old is admitted with chest pain, ST-segment changes, and fever. Troponin, CK-MB, and BNP are ordered to differentiate myocarditis from ACS. Results: troponin 2.4 ng/mL, CK-MB 18 ng/mL, BNP 310 pg/mL.

Correct coding: 84484 (troponin, quantitative); 82553 (CK-MB fraction); 83880 (natriuretic peptide); ICD-10-CM I40.9 (acute myocarditis, unspecified).

Why: Each analyte is a distinct reportable code; no NCCI PTP edit bundles these together [4]. Each requires its own medical necessity documentation in the order and clinical note.

Scenario 4: Post-CABG Troponin for Perioperative Cardiac Monitoring

On post-operative day 2 after CABG, a patient develops hypotension. The attending cardiologist orders a single troponin to evaluate for perioperative myocardial injury. Result: 4.1 ng/mL. No serial draw is ordered.

Correct coding: 84484 x 1 unit; ICD-10-CM I97.190 (other intraoperative cardiac functional disturbances) or applicable postoperative complication code. No Modifier 91 (single draw).

Why: The clinical note must document the specific concern prompting the order (hypotension with clinical concern for perioperative MI), not a generic "routine post-op labs" entry. Routine postoperative troponin without documented clinical indication does not meet medical necessity and is a recognized audit target for cardiac biomarker testing [10].


Related Codes

  • 84512: Troponin, qualitative; the sibling code for positive/negative result reporting
  • 82553: Creatine kinase (CK), MB fraction only; cardiac-specific CK marker commonly co-ordered in ACS or myocarditis workups
  • 82550: Creatine kinase (CK), total; general muscle enzyme, less cardiac specific than CK-MB or troponin
  • 83874: Myoglobin; early rising cardiac marker, ordered less frequently in modern ACS protocols
  • 83880: Natriuretic peptide (BNP/NT-proBNP); co-ordered in dyspnea workups to differentiate heart failure from ACS
  • 36415: Collection of venous blood by venipuncture; separately reportable specimen collection

Sources

  1. 2021 AHA/ACC Guideline for the Evaluation and Diagnosis of Chest Pain — ACC/AHA — Clinical guideline establishing hs-cTn as the preferred ACS biomarker and serial troponin protocols (Class I)
  2. AMA CPT Code Information — AMA — Official CPT code set authority for 84484 and 84512 descriptors and isoform scope
  3. CMS Claims Processing Manual, Chapter 16 — CMS — Laboratory billing rules, Modifier 91 requirements, medical necessity standards, and OPPS packaging policy
  4. CMS NCCI PTP Edits — CMS — Procedure-to-procedure edit tables, updated quarterly
  5. CMS NCCI Medically Unlikely Edits — CMS — MUE = 2 confirmed for 84484
  6. CMS Medicare Coverage Database — CMS — LCD/NCD search; no standalone NCD confirmed for troponin
  7. CMS Clinical Laboratory Fee Schedule — CMS — Annual CLFS payment rates for 84484; verify current-year rate
  8. CMS CLIA Program — CMS — CLIA certificate requirements; 84484 requires moderate or high complexity certification
  9. CDC CLIA Waived Test List — CDC/CMS — Verify waived status for any specific point-of-care troponin device before appending QW
  10. HHS OIG Compliance Guidance, Laboratory — HHS OIG — General laboratory compliance guidance; cardiac biomarker medical necessity and duplicate billing scrutiny areas

Related Codes

Official Description

Troponin, quantitative

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 84484 refers to a quantitative measurement of troponin levels in the blood. Troponins are a group of proteins found in cardiac and skeletal muscle that play a crucial role in muscle contraction by forming calcium bonds. Specifically, Troponin T is responsible for binding to tropomyosin, forming a complex that is essential for muscle function, while Troponin I binds to actin, stabilizing the Troponin T-tropomyosin complex. The measurement of troponin levels is particularly significant in the context of cardiac health, as elevated troponin levels, when accompanied by symptoms such as chest pain, are indicative of cardiac injury, including conditions like myocardial infarction (heart attack). This test is frequently utilized in emergency settings, especially in the Emergency Department, where it is often ordered for patients presenting with potential myocardial infarction. The troponin test is typically repeated at intervals of six hours to monitor changes in troponin levels over time. Additionally, it may be ordered alongside other cardiac biomarker tests, such as creatine kinase (CK), CK-MB, and myoglobin, to provide a comprehensive assessment of cardiac function. The blood sample for this test is obtained through a venipuncture, which is a separately reportable procedure. The analysis of the serum or plasma involves testing for Troponin T using a quantitative electrochemiluminescent immunoassay, while Troponin I is measured using a chemiluminescent immunoassay.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The troponin quantitative test (CPT® Code 84484) is indicated for the following conditions:

  • Myocardial Infarction The test is primarily ordered when a patient presents with symptoms suggestive of a heart attack, such as chest pain, shortness of breath, or other cardiac-related symptoms.
  • Cardiac Injury Elevated troponin levels are used to diagnose various forms of cardiac injury, which may include conditions beyond myocardial infarction.
  • Monitoring Cardiac Status The test may be repeated at six-hour intervals to monitor changes in troponin levels, providing critical information about the patient's cardiac status over time.

2. Procedure

The procedure for obtaining a troponin quantitative test involves several key steps:

  • Step 1: Patient Preparation Prior to the blood draw, the patient may be assessed for symptoms of cardiac distress, and their medical history may be reviewed to determine the necessity of the troponin test.
  • Step 2: Venipuncture A blood sample is collected through a venipuncture, which is a procedure where a needle is inserted into a vein, typically in the arm, to draw blood. This step is crucial as it provides the serum or plasma needed for testing.
  • Step 3: Sample Processing Once the blood sample is obtained, it is processed to separate the serum or plasma from the cellular components. This is essential for accurate testing of troponin levels.
  • Step 4: Troponin Testing The serum is then tested for Troponin T using a quantitative electrochemiluminescent immunoassay, while Troponin I is measured using a chemiluminescent immunoassay. These methods allow for precise quantification of troponin levels in the blood.

3. Post-Procedure

After the troponin test is performed, the patient may be monitored for any immediate reactions to the venipuncture. The results of the troponin test are typically available within a short timeframe, allowing for timely clinical decision-making. If elevated troponin levels are detected, further evaluation and management of the patient's cardiac condition may be initiated. It is important to note that the troponin test may be repeated at six-hour intervals to track changes in troponin levels, which can provide valuable insights into the progression or resolution of cardiac injury. Additionally, healthcare providers may consider the troponin results in conjunction with other cardiac biomarkers and clinical findings to formulate a comprehensive treatment plan.

Short Descr ASSAY OF TROPONIN QUANT
Medium Descr ASSAY OF TROPONIN QUANTITATIVE
Long Descr Troponin, quantitative
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
Q4 Service for ordering/referring physician qualifies as a service exemption
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
CR Catastrophe/disaster related
GA Waiver of liability statement issued as required by payer policy, individual case
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
GW Service not related to the hospice patient's terminal condition
X5 Diagnostic services requested by another clinician: for reporting services by a clinician who furnishes care to the patient only as requested by another clinician or subsequent and related services requested by another clinician; this modifier is reported for patient relationships that may not be adequately captured by the above alternative categories; reporting clinician service examples include but are not limited to, the radiologist's interpretation of an imaging study requested by another clinician
PD Diagnostic or related non diagnostic item or service provided in a wholly owned or operated entity to a patient who is admitted as an inpatient within 3 days
AY Item or service furnished to an esrd patient that is not for the treatment of esrd
CS Cost-sharing waived for specified covid-19 testing-related services that result in and order for or administration of a covid-19 test and/or used for cost-sharing waived preventive services furnished via telehealth in rural health clinics and federally qualified health centers during the covid-19 public health emergency
GC This service has been performed in part by a resident under the direction of a teaching physician
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
GZ Item or service expected to be denied as not reasonable and necessary
PO Excepted service provided at an off-campus, outpatient, provider-based department of a hospital
Q5 Service furnished under a reciprocal billing arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
RT Right side (used to identify procedures performed on the right side of the body)
SA Nurse practitioner rendering service in collaboration with a physician
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2013-01-01 Changed Medium Descriptor changed.
2011-01-01 Changed Short description changed.
1997-01-01 Added First appearance in code book in 1997.
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Description
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Description
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