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Official Description

Diagnostic bone marrow; biopsy(ies)

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

Diagnostic bone marrow biopsy, represented by CPT® Code 38221, is a medical procedure utilized to obtain samples of bone marrow for the purpose of diagnosing various hematological diseases and cancers. This procedure involves the extraction of bone marrow aspirate or tissue samples, which can be collected from multiple anatomical sites; however, the anterior or posterior iliac crest is the most frequently chosen location due to its accessibility and the quality of the samples obtained. In certain cases, such as aspiration only, samples may also be taken from the breastbone or, particularly in pediatric patients under 18 months, from the lower leg. The procedure typically begins with aspiration, which is performed first to collect liquid bone marrow. Following this, if a biopsy is indicated, a larger needle is employed to extract a cylindrical core sample of solid bone marrow tissue. The technique requires careful navigation to avoid surrounding nerves and involves specific maneuvers to ensure successful sample collection. The procedure is critical for diagnosing conditions such as leukemia, lymphoma, and other blood disorders, providing essential information for treatment planning and patient management.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

Bone marrow biopsy is indicated for the evaluation of various hematological conditions and diseases. The following are specific indications for performing this procedure:

  • Hematological Disorders Diagnosis of blood disorders such as anemia, thrombocytopenia, or leukopenia.
  • Cancers Assessment of hematological malignancies, including leukemia and lymphoma.
  • Bone Marrow Disorders Investigation of conditions affecting bone marrow function, such as myelodysplastic syndromes or aplastic anemia.
  • Staging of Cancer Determining the extent of cancer spread, particularly in cases of known malignancies.

2. Procedure

The procedure for a diagnostic bone marrow biopsy involves several critical steps to ensure accurate sample collection. The following outlines the procedural steps:

  • Step 1: Patient Preparation The patient is positioned comfortably, and the area over the iliac crest is cleaned and sterilized to minimize the risk of infection. Local anesthesia may be administered to numb the area where the biopsy will be performed.
  • Step 2: Aspiration A hollow needle attached to a syringe is inserted into the iliac crest, typically advancing about 4-5 cm. The clinician must take care to avoid the lateral femoral cutaneous nerve when accessing the anterior iliac crest or the cluneal nerves when accessing the posterior iliac crest. The needle is advanced using firm pressure and an alternating motion or taps with a mallet to facilitate entry into the bone marrow cavity.
  • Step 3: Sample Collection Once the needle is in place, the plunger of the syringe is pulled back to aspirate a sample of liquid bone marrow. If no aspirate is obtained, the needle may be repositioned to collect additional samples. Multiple aspirations may be performed to ensure adequate sample collection.
  • Step 4: Biopsy Following aspiration, if a biopsy is required, a larger needle designed for core sampling is introduced either through the same incision or a new one. This needle is redirected to extract a cylindrical core of solid bone marrow tissue. The site is then bandaged to promote healing.

3. Post-Procedure

After the bone marrow biopsy, patients are typically monitored for any immediate complications, such as bleeding or infection at the biopsy site. It is common for patients to experience some discomfort or soreness in the area for a few days following the procedure. Instructions for post-procedure care may include keeping the biopsy site clean and dry, avoiding strenuous activities for a short period, and monitoring for any signs of complications. Follow-up appointments may be scheduled to discuss the results of the biopsy and any further necessary treatment.

Short Descr DX BONE MARROW BIOPSIES
Medium Descr DIAGNOSTIC BONE MARROW BIOPSIES
Long Descr Diagnostic bone marrow; biopsy(ies)
Status Code Active Code
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 0 - Physician Service Code
Multiple Procedures (51) 2 - Standard payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50) 1 - 150% payment adjustment for bilateral procedures applies.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 0 - Payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62) 0 - Co-surgeons not permitted for this procedure.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Hospital Part B services paid through a comprehensive APC
ASC Payment Indicator Office-based surgical procedure added to ASC list in CY 2008 or later with MPFS nonfacility PE RVUs; payment based on MPFS nonfacility PE RVUs.
Type of Service (TOS) 2 - Surgery
Berenson-Eggers TOS (BETOS) P6C - Minor procedures - other (Medicare fee schedule)
MUE 1
CCS Clinical Classification 65 - Bone marrow biopsy

This is a primary code that can be used with these additional add-on codes.

77002 CPT Add On MPFS Status: Active Code APC N ASC N1 Physician Quality Reporting CPT Assistant Article Fluoroscopic guidance for needle placement (eg, biopsy, aspiration, injection, localization device) (List separately in addition to code for primary procedure)
RT Right side (used to identify procedures performed on the right side of the body)
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
LT Left side (used to identify procedures performed on the left side of the body)
X5 Diagnostic services requested by another clinician: for reporting services by a clinician who furnishes care to the patient only as requested by another clinician or subsequent and related services requested by another clinician; this modifier is reported for patient relationships that may not be adequately captured by the above alternative categories; reporting clinician service examples include but are not limited to, the radiologist's interpretation of an imaging study requested by another clinician
X4 Episodic/focused services: for reporting services by clinicians who provide focused care on particular types of treatment limited to a defined period and circumstance; the patient has a problem, acute or chronic, that will be treated with surgery, radiation, or some other type of generally time-limited intervention; reporting clinician service examples include but are not limited to, the orthopedic surgeon performing a knee replacement and seeing the patient through the postoperative period
22 Increased procedural services: when the work required to provide a service is substantially greater than typically required, it may be identified by adding modifier 22 to the usual procedure code. documentation must support the substantial additional work and the reason for the additional work (ie, increased intensity, time, technical difficulty of procedure, severity of patient's condition, physical and mental effort required). note: this modifier should not be appended to an e/m service.
50 Bilateral procedure: unless otherwise identified in the listings, bilateral procedures that are performed at the same session, should be identified by adding modifier 50 to the appropriate 5 digit code. note: this modifier should not be appended to designated "add-on" codes (see appendix d).
51 Multiple procedures: when multiple procedures, other than e/m services, physical medicine and rehabilitation services or provision of supplies (eg, vaccines), are performed at the same session by the same individual, the primary procedure or service may be reported as listed. the additional procedure(s) or service(s) may be identified by appending modifier 51 to the additional procedure or service code(s). note: this modifier should not be appended to designated "add-on" codes (see appendix d).
52 Reduced services: under certain circumstances a service or procedure is partially reduced or eliminated at the discretion of the physician or other qualified health care professional. under these circumstances the service provided can be identified by its usual procedure number and the addition of modifier 52, signifying that the service is reduced. this provides a means of reporting reduced services without disturbing the identification of the basic service. note: for hospital outpatient reporting of a previously scheduled procedure/service that is partially reduced or cancelled as a result of extenuating circumstances or those that threaten the well-being of the patient prior to or after administration of anesthesia, see modifiers 73 and 74 (see modifiers approved for asc hospital outpatient use).
53 Discontinued procedure: under certain circumstances, the physician or other qualified health care professional may elect to terminate a surgical or diagnostic procedure. due to extenuating circumstances or those that threaten the well being of the patient, it may be necessary to indicate that a surgical or diagnostic procedure was started but discontinued. this circumstance may be reported by adding modifier 53 to the code reported by the individual for the discontinued procedure. note: this modifier is not used to report the elective cancellation of a procedure prior to the patient's anesthesia induction and/or surgical preparation in the operating suite. for outpatient hospital/ambulatory surgery center (asc) reporting of a previously scheduled procedure/service that is partially reduced or cancelled as a result of extenuating circumstances or those that threaten the well being of the patient prior to or after administration of anesthesia, see modifiers 73 and 74 (see modifiers approved for asc hospital outpatient use).
58 Staged or related procedure or service by the same physician or other qualified health care professional during the postoperative period: it may be necessary to indicate that the performance of a procedure or service during the postoperative period was: (a) planned or anticipated (staged); (b) more extensive than the original procedure; or (c) for therapy following a surgical procedure. this circumstance may be reported by adding modifier 58 to the staged or related procedure. note: for treatment of a problem that requires a return to the operating/procedure room (eg, unanticipated clinical condition), see modifier 78.
79 Unrelated procedure or service by the same physician or other qualified health care professional during the postoperative period: the individual may need to indicate that the performance of a procedure or service during the postoperative period was unrelated to the original procedure. this circumstance may be reported by using modifier 79. (for repeat procedures on the same day, see modifier 76.)
80 Assistant surgeon: surgical assistant services may be identified by adding modifier 80 to the usual procedure number(s).
82 Assistant surgeon (when qualified resident surgeon not available): the unavailability of a qualified resident surgeon is a prerequisite for use of modifier 82 appended to the usual procedure code number(s).
95 Synchronous telemedicine service rendered via a real-time interactive audio and video telecommunications system: synchronous telemedicine service is defined as a real-time interaction between a physician or other qualified health care professional and a patient who is located at a distant site from the physician or other qualified health care professional. the totality of the communication of information exchanged between the physician or other qualified health care professional and the patient during the course of the synchronous telemedicine service must be of an amount and nature that would be sufficient to meet the key components and/or requirements of the same service when rendered via a face-to-face interaction. modifier 95 may only be appended to the services listed in appendix p. appendix p is the list of cpt codes for services that are typically performed face-to-face, but may be rendered via a real-time (synchronous) interactive audio and video telecommunications system.
AG Primary physician
AQ Physician providing a service in an unlisted health professional shortage area (hpsa)
AS Physician assistant, nurse practitioner, or clinical nurse specialist services for assistant at surgery
CC Procedure code change (use 'cc' when the procedure code submitted was changed either for administrative reasons or because an incorrect code was filed)
CR Catastrophe/disaster related
GA Waiver of liability statement issued as required by payer policy, individual case
GC This service has been performed in part by a resident under the direction of a teaching physician
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GW Service not related to the hospice patient's terminal condition
MG The order for this service does not have applicable appropriate use criteria in the qualified clinical decision support mechanism consulted by the ordering professional
MH Unknown if ordering professional consulted a clinical decision support mechanism for this service, related information was not provided to the furnishing professional or provider
PO Excepted service provided at an off-campus, outpatient, provider-based department of a hospital
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
QQ Ordering professional consulted a qualified clinical decision support mechanism for this service and the related data was provided to the furnishing professional
SA Nurse practitioner rendering service in collaboration with a physician
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XP Separate practitioner, a service that is distinct because it was performed by a different practitioner
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2018-01-01 Changed Long, medium and short descriptions changed. AMA guidelines changed.
2013-01-01 Changed Guideline information changed.
2003-01-01 Changed Code description changed.
2002-01-01 Added Added.
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