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Official Description

Semen analysis; presence and/or motility of sperm including Huhner test (post coital)

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The semen analysis, specifically identified by CPT® Code 89300, is a laboratory procedure that assesses the presence and motility of sperm, including the Huhner test, also known as the postcoital test. This test is performed to evaluate the interaction between a woman's cervical mucus and her partner's sperm during the ovulation period. The procedure is typically conducted when laboratory tests indicate that ovulation is imminent. To prepare for the test, the couple is advised to abstain from sexual intercourse for a period of two days. Following this abstinence, they are instructed to engage in intercourse and then present to the physician's office approximately six to ten hours later for the analysis. During the test, a small sample of cervical mucus is collected from the endocervix and examined microscopically. The analysis focuses on two primary factors: the presence of sperm within the mucus and the motility, or movement, of the sperm. A favorable outcome of the postcoital test is characterized by a significant number of active sperm exhibiting good forward motion through the cervical mucus, indicating a potentially conducive environment for fertilization. Conversely, an unfavorable result is indicated by either a low sperm count or a lack of active sperm demonstrating effective motility, which may suggest issues with fertility that warrant further investigation.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The semen analysis, CPT® Code 89300, is indicated for the following conditions:

  • Evaluation of Fertility Issues This test is performed to assess potential fertility problems in couples who are experiencing difficulty conceiving.
  • Assessment of Sperm Function The procedure evaluates how well sperm can move through the cervical mucus, which is crucial for successful fertilization.
  • Timing of Ovulation The test is conducted when laboratory testing indicates that ovulation is imminent, allowing for optimal timing of intercourse.

2. Procedure

The procedure for the semen analysis, CPT® Code 89300, involves several key steps that ensure accurate evaluation of sperm presence and motility.

  • Step 1: Preparation for the Test The couple is instructed to abstain from sexual intercourse for a minimum of two days prior to the test. This abstinence is crucial as it allows for an adequate buildup of sperm, which is necessary for a comprehensive analysis.
  • Step 2: Intercourse After the two-day abstinence period, the couple engages in sexual intercourse. This step is essential as it allows for the collection of sperm in the cervical mucus during the ovulation window.
  • Step 3: Sample Collection Approximately six to ten hours after intercourse, the woman presents to the physician's office. A small sample of cervical mucus is then collected from the endocervix. This sample is critical for the subsequent analysis of sperm presence and motility.
  • Step 4: Microscopic Examination The collected mucus sample is examined under a microscope. The evaluation focuses on two main aspects: the presence of sperm within the mucus and the motility of the sperm. The technician assesses whether there are a significant number of active sperm and their ability to move effectively through the mucus.

3. Post-Procedure

After the semen analysis is completed, the results are typically reviewed by the physician. The outcomes of the postcoital test can provide valuable insights into the couple's fertility status. A favorable result, characterized by a high number of motile sperm, may indicate a conducive environment for fertilization, while an unfavorable result may suggest potential fertility issues that require further investigation or intervention. Patients may be advised on next steps based on the findings, which could include additional testing or treatment options to address any identified concerns.

Short Descr SEMEN ANALYSIS W/HUHNER
Medium Descr SEMEN ALYS PRESENCE&/MOTILITY SPRM HUHNER
Long Descr Semen analysis; presence and/or motility of sperm including Huhner test (post coital)
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) Yes
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
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