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Official Description

Semen analysis; volume, count, motility, and differential

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 89320 refers to a semen analysis that encompasses a comprehensive evaluation of several key parameters: volume, count, motility, and differential. This analysis is crucial for assessing male fertility and involves measuring the total volume of semen produced in a single ejaculation, which provides insight into reproductive health. The sperm count is determined by calculating the total density of sperm per milliliter of semen, with a normal threshold set at 20 million sperm per milliliter or more. Additionally, motility is assessed to determine the number of active sperm that exhibit good forward motion, which is essential for successful fertilization. A normal motility count is defined as 8 million motile sperm per milliliter or higher. Furthermore, the differential analysis evaluates the morphology of sperm, identifying the proportion of sperm that appear normal when examined microscopically. This comprehensive semen analysis is vital for diagnosing potential fertility issues and guiding further reproductive health interventions.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The semen analysis coded under CPT® 89320 is indicated for various clinical scenarios related to male fertility assessment. The following conditions may warrant this procedure:

  • Infertility Evaluation This analysis is performed when a couple is experiencing difficulties conceiving, allowing for the assessment of male reproductive health.
  • Post-Vasectomy Assessment Following a vasectomy, this test may be conducted to confirm the absence of sperm in the semen, indicating the success of the procedure.
  • Assessment of Sperm Quality This analysis is utilized to evaluate the quality of sperm in cases where there are concerns about sperm health, motility, or morphology.
  • Hormonal Imbalance Investigation In cases of suspected hormonal issues affecting fertility, a semen analysis can provide valuable information regarding sperm production and function.

2. Procedure

The procedure for conducting a semen analysis under CPT® 89320 involves several critical steps to ensure accurate results. The following outlines the procedural steps:

  • Step 1: Sample Collection The first step involves the collection of a semen sample, typically obtained through masturbation into a sterile container. It is essential that the sample is collected after a period of sexual abstinence, usually 2 to 7 days, to ensure optimal sperm concentration.
  • Step 2: Volume Measurement Once the sample is collected, the total volume of the semen is measured. This measurement is crucial as it provides insight into the overall reproductive health of the individual.
  • Step 3: Sperm Count Determination The next step involves calculating the sperm count, which is the total number of sperm present per milliliter of semen. This is performed using a hemocytometer or an automated sperm analyzer, with a normal count being 20 million sperm per milliliter or more.
  • Step 4: Motility Assessment Following the count, the motility of the sperm is evaluated. This involves assessing the percentage of sperm that are actively moving and their forward progression. A normal motility count is defined as 8 million motile sperm per milliliter or higher.
  • Step 5: Differential Analysis Finally, a differential analysis is conducted to evaluate the morphology of the sperm. This step involves examining the sperm under a microscope to determine the percentage of sperm that appear normal in shape and structure.

3. Post-Procedure

After the semen analysis is completed, the results are compiled and interpreted by a qualified healthcare professional. Patients may be advised to discuss the findings in a follow-up appointment to understand the implications of the results on their fertility. Depending on the outcomes, further testing or treatment options may be recommended. It is also important for patients to be aware that factors such as abstinence duration, sample handling, and laboratory techniques can influence the results of the semen analysis.

Short Descr SEMEN ANAL VOL/COUNT/MOT
Medium Descr SEMEN ANALYSIS VOLUME COUNT MOTILITY DIFFERENT
Long Descr Semen analysis; volume, count, motility, and differential
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 206 - Microscopic examination (bacterial smear, culture, toxicology)
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
Q4 Service for ordering/referring physician qualifies as a service exemption
Date
Action
Notes
2013-01-01 Changed Guideline information changed.
2008-01-01 Changed Code description changed.
Pre-1990 Added Code added.
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